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However, nickel affinity purifications are frequently used as a first step in an overall purification protocol that might also include ion-exchange, hydrophobic-interaction, or size-exclusion steps

10.1016/bs.ctdb.2015.07.016 Curr

*Correspondence: Hongliang Zhang, [email protected] Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers